For Release 2001/03/07 : CIA-RDP96-00792ROO0500240008-9 C 14 INTERACTIO NS WITH SCALAR ENERGY- CELLULAR KECHANISKS OF ACTION Dr. Glen Rein U.S.A. ;A, -enhanced pec@,,.nt direct experimental data as well as sophisticated CCcVuter Mathematical analyses of pre-existing data has revealed the Predominance- of rm-linear processes in biological systems (1) . Maese new findings are a dramatic departure from traditional. theories which indicate that . biochemical reactions are at or rear a state of equilibrium and that cells fumtion in a linear, dispersive and degenerative manner. Electrochemical csci.1lations betwm membrw-.-_bcund lipids near phassA-transition tenireratures have been reanalyzed with the aid of paoerful c=pxters and sha@ to be most a=,--ately des=ibed by nonlinear quantum mechanical equations(2). Similar analyses of coupling between barmanic oscillators represented by action potentials generated f3= active neuronal networks th.-central.. nervous system has revealed their non-linear nabxm(3). Sevexal types of quasi-particles, each with their -own frequencies, have been proposed; to radiate these non-linear rescnant - tons and plasmons. Biological solitons phermanon, including solitons, evA recpAved the rost attenticn, and have been postulated to be carriers have. of j)iologjcaj informaticn along ma nolecules Like. M(4) and alpha-helical intr@arane proteins involved with signal trarmIuction mectmnisms (5) . R=thermore, solitcns have, bow postulated to radiate the ocj@ electmuc e=itations associated with the action of ultra-violet and visible light on biological rystem(6) and the calcim-dependent cocperative interactions betdawn ectraoallular glyccproteins on the surface of the Plasma membrane in response to weak WX electromagnetic: (EM) radiation(5). In light of the non-lbiear nature of biological systems, it seems likely that they should be highly sensitive to non-linear forms of in the environment and that the acoustic and electromagnetic radiation riglit fre_@es of non-linear energies might have profound healing m-ties. Emgenous non-linear energies were first utilized by Nicholas PrOl he ado Springs experiments where Tesla in 1899 in his famous Color demmstrated the transmission of his lqesia waves" viU=& energy loss (7). 7 waves haw. since been studied in nif A n=Hiertzian od@ics, geology and astrt@ysics. Me extraction of scalar hydi- from Maxwell's original electxca@gnetic equations(8,9) and frcm ccrqxments Scinodinger's equations using imagimy rumters(10) has recently been reported. mie unusual properties of these non;-1bmr waves have also been -described by other investigators (8,11,12). Ha4ever, relatively little attumtion has been given to their effects on biological system. Due to the non-linear nature of biological systems. it was PrCp0sed that scalar wavw should be more biologically active than their linear electromagnetic COUnterpaxts(13). A recent report by the author proposed a new theory, the cry,-talline Transduction Theory, to explain the mechanism of action of scalar waves with biological systems. It was Proposed that scalar eXMW is txansduced into linear elecU=agnetic energy in the body by liquid crys. .,tals in the oell membrane and solid crystals found in the blood and in several biological tissues. Direct effects of scalar energy and coupling @pproved For Release 2001/03/07 : CIA-RDP96-00792ROO0500240008-9 Approved For Release 2001/03/07: CIA-RDP96-00792ROO0500240008 -etic fields was also ,0 conventional electromagr .gy t z or scalar ene considered(14). reliable scalar generators for n1p dIfficulty of cbtam@ ical studies has led to a paucity of hard exPer'mntal data*Hawever' biolog @ @il:::h succes@:@ly a&Vted 'the "mcbius striplo tAXJ=lOgy for r)r. Andile scalar waves to a watch. E17 a=m Tnt- has recently modified genexat Is design and now markp a readily and impoved Dr. Puharich lity of available, scalar W)eratOrl the Tesla watch. Despite the aw'flabiion Of such a scalar generator little is la=n abcllt thp- mech@ 0 scalar energy with biological systems. Nunprous anAxdot,i clinical cbsezvations of the healing prcperties Of the Tesla. watch bave been reported. Since several of thesee reports include imidences where- dinimition of the vyq@t=logy was correlated with frm watch ea. e=ired batteries) cessation of the scalar em"ions . the ikely tO without t1'e knwlec3ge Of the subject, placebo effects are un' explaln the clJJUcal results. Jjc@@ess' it Is unlax= whether the -ed ti=2e in the body or whether the 'calar fields directly effect disea direct effect of scalar energy Clinical effects are mediated thr@ a is involved it is likely that various the brairVbind. if the brairVuind or beliefs) will alter the psychological states(Og. stress, moods -biological effects Of. the scalar energy* C=CML S==S w= SCAIMTHEMY expwjwnts with int icat. ,,@r@tic,, of clinical one These variables c=1P1 variables, the scalar energy. In additcn to conventional confOurding explain why scW must -11w consider the Em environment- Mese factors may -n indivLd.ials are more sensitive to scalar er@ than others, as has bee previ(xmlY observed with EK energy- Even when individuals who are sensitive to scalar energy are pmWterm@ and isolated fY the rest of ariable response tO @ion Q, ,r st,@, they show a highly V im the pqulat treatment. n- udy the heal 3 ,ese problems Make it difficult to sb setting. properties of scalar (or EM) eljergies, in a clinical EVIrEt;= FM = BIOWICAL OF SQUAR ENEMY Dr.Eldon Byrd studyed the effects of scalar er*ZW on brain V"Os by wring a Vatch(3-5) - nw, results subjects EW before and after % with a preferential aninCreaSed a@lit of all EEr. frequencies effeat in the lower EV frMfflenCiesf In an J!IVC@ Of wearing the watch bx1icating the scalar 82 Hz was nOt seen in subjects the mvl=@, t"e wave neutralized this EH signal in indivi&3al. It Is Wtrx%qM whether tl@ altexed brain state is the actual cause of clinical healing which scmetimes" occurs several =ntbs after initial exposure to the scalar waves. It is kxxm that the brain and the body, eg. Q jamm sy@, ==nicate bi di rectiWally (16) . Thus, a di" _ bocly Muld cause a stlseq@ change Jy on thp action 'of scalar merc inidcate uhather in brain states. nwxefore these EM studies do not t1w brain and the scalar. enerW also ef f ects the body directly Or 114@ body are both effected. UM TLSSUE cuL= APPROACH -2- Approved For Release 2001/03/07: CIA-RDP96-00792ROO0500240008-9 pproved For Release 2001103/07 : CIA-RDP96-00792ROO0500240008-9 use of cultured cells, separated fmm the body and therefore isolated (at least to a certain degree) from the influeno,--s of the mind offer a unique experimental approach to Undlerstan@g the medial@ Of action of scalar energy in the healing process. Miis approach could be used to determine direct effect of scalar energy on cells frOM either the brain or the body. Tissue culture cells are a stable, ca-A=Jable bioloqical preparation which could also be used to study the biochwacal meclumism of action of scalar energy. Even if scalar energy directly effa,-ts individual cells, it may AIM effect the mind resulting in an altered psychological outlook which results in clinical inprovement. Dr. Persinger was the first person to test the effects of scalar energy using isolated cells(17). The scalar waves Were generated by Partially cancelling two vortex-type magnetic fieldr.(O.Miz, I0uT) by intersecting them in air. Although a scalar. field is likely to be generated in this prot(=l, it was n:;rt measured. The biological endpoint, degranulation of mast calls, was jr4z-eased by the scalar fields. It is unknown to what extea-It the EK fields contribute to,.this result.. Very recently, Dr.Puharich has reported that the.scaliLi' energy from the Tesla watch effects the UIA of E.'Coli cells gra-M in tissue culture(18) " Worku-q with colleagues at the Max Planck Bistibzte in Germany, they have -shown, that.the PAD-6 gene is activated, -by..scalar ene:rgy resulting in in=eased activity of ubiquitone. Ubigaftcne is a prorte3n involved in EM repa:Ir. M-,ese results indicate tlicit scalar energy can bave a direct effect at the suboallular level as well as a direct effect on the Im" system (mast cells). EMrT OF sauM MMW CN M9Z=RMM= RN=CU -nm present study is the first to report a direct effect of energy on nerve calls In t:issue culture and indicates that scalar energy can imdulate the basic'biochemical podbunication between nerve cells mediated by nizurvtransmitters. 1*-rve cells were chosen for this- study to determine whether scalar erergy can directly effect the nervous system in the absence of any feedback signals from the body. Cultured nerw cells offer a unique model to answer this questicn. A particular type Of nerv8 cell, called PC22 cells, originally isolated from a rat adrenal phaeochrcmoqytcma, was used because its neurotransmitter prcperties have been well characterized and shown to be. similar to. t=e o=wing during normal vffzptic transmission in the brain (3.9,20). Furdwxmore, the author has previcusly shown that the fm-cticnai properties of noradrenaline, an abundent neurotransmitter in these oUls is altered by weak EK fields. Nor-ddrenaline release Fr Pc12 cel-Is was shown to be increased by a 500HI mcnopolar square wave(21) and r=adrenalim uptake was inhibited by a 3-5 Hz bipolar s9lare, wave (22). Noradra-aline uptake was chosen as the functional neurotransmitter property for this study for several reasons. Hourotransmitter uptake is directly mediated by the plasma meribrane via an Mtrmembrane protein carrier. 2v-- plasma membrane, with its liquid-=Istal str@, is the critical cellular barrier which in@faces with the chemical and electrcmagnetic environment. since other xtram=brane proteins show non-lh-ear properties, the plasma m=brane appears to be a likely target for scalar energy. Although neurotransmitter releaze also involves the plasma me:rbrane, it is a mxh more ocaplicated PrOceSs Which Occurs several biochemical steps down the line from the first exposure of the membrane to theEM signal. -3- Approved For Release 2001/03/07 : CIA-RDP96-00792ROO0500240008-9 Approved For Release 2001103107 : CIA-RDP96-00792ROO0500240008-9 The Ptqsiological function of carrier radiated neuro@smitter uPtake is to remove ex:cess neurotranismitter from the synaptic cleft after its ralease from pmsyna@c nerve,. M=, zhibiting uptake results in the accumulation of the nelrotransmitter the Syrk in VUC cleft. This is the rediani= of action of tricyclic antidepressants, r .ir Jce the Mlradmllaline: uptake-Depres-sion is associated with decreased _y inhibit rcradrenalinelevels. F= these studies, the same Protocol which was PrWiOusly used to deter,=-.e- thp- effects Of electr@etic fields on noradrenaline uptal was used(22). Mlis involved exposing caxfluent ranolayer culture _ of PC12 Ce@ to radiolabelled noradmmlUv__ in tim prese@ Or abserm Of scalar enexgy. After a 30 minute eypcsure pexiod, thn. cells were washed and centr3.fuged according to st-:u@ biochemical prcced:ures and the am=-it of mBerml =dmnnac1=U'tP_ that was takp-n uP fto the cells was WaSUred, Ulin; a illatlcn =zter. qhe results were M=al,zed w1th respect to the tctal intracellular Protein Concentration as deterMineid by standard biochemical mthods using.a spectz results are expressed as cpm/n 'PPhOtc@er- Me final specific activity -q prbtein. Mle Tesla watch obtained frcra ELF Coo=m'- source - Intenaticnal was used as a of 'calar mergy- According to I)r-Rtlarich tII-- scalar B field -emitted by.the watch is-around 8..Hz. The-Tesla watch (digital. versicn). Was Placed directly over an Uncovered Petri dish containin@ confluent PC22 C*-Ils- At tbe sc are time a sepamte control dish, placed aba@ :L2 fee _t frcm the e5q)er@al dish' was OP)Sed In an identical way to a watch that did not have the scalar gen-Zat3lIg WbJUS Str:Lp. In this way both cmi:trol and exParimental dishes received the same electr@etic field wcpcsure,, bt the experimental dishes vex-- also exposed to a scalar f ield. indicated in T The, results are able 1. Overall, uptake Of roradrenalin. into PC12 cells was irIlibited by 19.5% in the presence of the Tesla watch as cmpared'with the control value Obtained in the absence of a scalar dfleld' Stan@ard t-test stat'st3-cal analysis revealed a highly sigruf.Icant ifference betunen control and experimental dishes of p, 0.01, Table i EFE= OF SCAIAR ENEMY CMVa= UpM M CH VCRA CCINTROL EXPER IMEUM (Ppmlng protein) (CM/mg protein) 10min . 30min 10min 30min lin 4065 1079 3500 2. 880 7032 1237 5009 3. 1076 4520 1110 3592 4. 9033 6035 5. 4277 4041 4117 M ean 1022 5507 1109 4435 S.E.-H.. + 17 +480 + 72 +841 These results eLUow us to ccmlude that the scalar erargY has a dirwt effecton nerve cells. The L nhibition of uptake is tun dqmndlent with n, significant effect being observed after a 10 mirzte exposure. it is -4- Approved For Release 2001103107 : CIA-RDP96-00792ROO0500240008-9 d For Release 2001/03/07 : CIA-RDP96-00792ROO0500240008-9 tr@=wn whetber exposure I- greater than 30 mbmte:s would produce a larger effect or whether the effect w:xald be maintained eitherin the 7=1timied presence of the scalar field or not. Ojese questions will be exmired in additional experiments. Since inhibition of uptake occured after only 30 Minutes, it is likely that this effect is an early biolcxjical response to scalar erjergy and may be the trigger for subsequent 11viological changes that result in inprovement of a wide variety of clinical diseases. Sinoe the scalar energy inhibited the uptake process, it is acting like a traditional antidepressant &I.V, thereby explaining the antidepressant action of the Tesla watch experienced by som injividuals. These results also indicate that scalar erergy, like electrcroagnetlc o.f, energy, can have a direct effect an the call m=brane. Since both scalar and electranagnetic energy. bave thi5- same effect on neurotransmitter rt. uptala!, the results offer indirect support for the crystalline TransductiOn theory- 7hus it is Possible that the scalar energy could be o=ez ted to elec;tr=agnetic eneergy in the membrane and the latter actually causes the biological effect. H7.,?eVer it is also possible that the scalar have a direct effect on the uptake system. Needless say in the presence a, of,e1ectrcmagneUc fields in biological X" - it.will-be-difficult.to rule cut. this.possibility. However, certain exper=ental approaches could be used to dete=dne the mechanism of action of the two types of energies. - eg.dete=ine whp _ther alterations j*,In the liquid crystal stn@cture in the mmixam would dnnge the effect of ''the scalar energy cn uptake. In light of the previous disct=ion, it Is also possible that the scalar energy will effect other calls in the body in addition to nerve Cells- MP- tissue cultze approach will be an invaluable tool in detemming to utiat a&-ent tl= is true. Mis approach could also be used to find out whether diseased cells are preferentially sensitive to scalar enexW- 7he tissue culture approach is thereom a valuable method for studying the biological effects of scalar energy and for ultimately characterizing its mechanism of action. 1) " Kau inear ElectrOdYnamics Jn Biological Systems",, Adey,,W.R. lawrence, A.F. (eds.),Plenum Press, N.Y. (1984). 2) Loor-ley-Millman,M.E.ert al. Biophysics J. M 221 .(1982) 3) Basar,E. American J.Physiol. 245 R510 .(1983) 4) Barlow,C-Y-et al. Nature 332 312 (1988). .5) Tawrvnce,A.F.,Adey,W.R. Na=log.Res. .4 3.15 (1982). 6) Becli*er,J.Internat.i.Radiation.Biol. a 147 (1985). 7) Tes:La,N.Olcolorado springs Notes" NOljt,Bejgradp,Terazjje 27,Yugoslavia. 8) Ai&': Biological Warfare, Bearden,T.E.,Tesla Book Co.,Tewcas (1988) 9) Bass,R. Proc.Tesia Syqposium,ps9,jntermt.Tesja Soc.,colorado Springs. (19 8 4) 10)R=qcherjE.A.,,Technic Res.1ab Peport PSPL-3jo7A,san T-eoandro, CA. 3.1)QUantUM MChanics and Nonlinear Waves, Dart, P. B..,Harwood Acad.N.Y. (1981) 12)Bearden,T.E. PACE Newletter.2(4)plO(1982). 13) Byrd,E.A. 35th ACUM OMf -Abstracts &218, Sept. (1982). 14)Rein,G. in 115achanisms of Psychic PerceptioWI,MUlay,J.,Sirag,S-P.(eds) in press. I5)By=I,E.A. in ELF cocoon Internat-catalogm,st-Frarrisvi3-ie,n. (1987). 16)"Pwy&zr,euroim=wlogy-1, Ader,R. (ed.),Academic Press,N.Y. (3-9sj). -5- Approved For Release 2001/03/07 : CIA-RDP96-00792ROO0500240008-9 Approved For Release 2001/03/07 : CIA-RDP96-00792ROO0500240008-9 17)see Byr:i,E.A. Archaeus icurnal .1 (I)pl (1983). 18)pubarich,A. 14th Ann.USPA omf.cn Bioenergy,Daytcn,Chio (1988). 19)Greem,:rj.A.,Rein,G. Nature 268 349 (1977). 20)Greene,:L.A.,r,ein,G. Brain P4search 138 521 (1977). 21)Dixey,R.,Rein,G. Nature 296 253 (1982). 22)Rein,G.,et al..Proc.9th Ann. Bioelectr@. Soc. Oregm (1987). -6- Approved For Release 2001/03/07 : CIA-RDP96-00792ROO0500240008-9